Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
نویسندگان
چکیده
Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
منابع مشابه
Molecular Evidence on Changing Pattern of Mixed Plasmodium falciparum and P. vivax Infections during Year-Round Transmission of Malaria in Chahbahar, Iran
Mixed malaria infections, Plasmodium falciparum and P. vivax, are suspected to occur at a greater frequency than is detected by conventional light microscopy. In order to determine the year round pattern of transmission and the frequency of mixed infections in malaria endemic area, we carried out a prospective comparison of diagnosis by conventional light microscopy and nested PCR in Chahbahar ...
متن کاملClinical Pharmacology of the Antimalarial Chloroquine in Children and Their Mothers
Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, and Plasmodium knowlesi are the parasites that infect humans. Plasmodium falciparum and Plasmodium vivax cause most of the malarial infections worldwide. Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, and Plasmodium knowlesi are susceptible to chloroquine. Chloroquine was the world's most widely used antim...
متن کاملWolbachia Infections Are Virulent and Inhibit the Human Malaria Parasite Plasmodium Falciparum in Anopheles Gambiae
Endosymbiotic Wolbachia bacteria are potent modulators of pathogen infection and transmission in multiple naturally and artificially infected insect species, including important vectors of human pathogens. Anopheles mosquitoes are naturally uninfected with Wolbachia, and stable artificial infections have not yet succeeded in this genus. Recent techniques have enabled establishment of somatic Wo...
متن کاملDetection of plasmodium falciparum infection in anopheles mosquitoes from Keonjhar district, Orissa, India.
The determination of the presence of malaria sporozoites in wild caught Anopheles mosquitoes remains an integral component in understanding the transmission dynamics in area-specific malaria epidemiological studies1. Mosquito infection rate, together with concurrent human-landing density data provide vulnerable parameters for estimating the intensity of transmission and entomological inoculatio...
متن کاملMalaria transmission-blocking activity in experimental infections of Anopheles gambiae from naturally infected Plasmodium falciparum gametocyte carriers.
Experimental infections of anopheline mosquitoes were carried out with Plasmodium falciparum gametocytes from 65 naturally infected patients in Cameroon. A comparison was made between infections with blood containing autologous plasma and blood in which the plasma was replaced with plasma from a donor without previous malaria exposure. A lower infection rate was observed in 50 of 65 autologous ...
متن کامل